Izici Zomkhiqizo
1. Ipuleti le-ELISA elitholakalayo elingama-96.
2. Ukuhlanza kalula ngesakhiwo esikhethekile esingaphansi.
3. Khetha indawo engaphezulu ngokuya ngosayizi wesisindo samangqamuzana amaprotheni kanye ne-hydrophobicity yamaprotheni.
● Ipuleti le-ELISA eline-adsorptive kakhulu: ukukhangiswa okuphezulu kwama-antibody-antigens wesisindo samangqamuzana angaphezu kuka-50kDa.
● Ipuleti le-ELISA eline-adsorptive elimaphakathi: i-adsorption engacacisiwe ngezansi, ingemuva eliphansi.
4. Khetha imibala ehlukene yamapuleti e-ELISA ngokwezindlela zokutholwa.
Amapuleti asobala - ukutholwa kombala; amapuleti amhlophe - ukutholwa kwe-luminescent; amapuleti amnyama - ukutholwa kwe-fluorescent.
1. Iyunifomu ngokuqina kanye nobubanzi bomthombo, kanye ne-orthoscopic phansi.
2. Ukubekezelelana okuncane phakathi kokugijima naphakathi kokugijima.
3. Maka yonke imithombo ngohlamvu oluhlukile nenombolo ukuze kube lula ukuhlola.
4. Amapuleti e-ELISA anokusebenza okuhlukile kwendawo angenziwa ngokwezifiso ngokuya ngezidingo zamakhasimende.