Ubuhlalu obuzibuthe busetshenziswa kakhulu ekuxilongeni amasosha omzimba, ekuxilongeni kwamangqamuzana, ekuhlanzweni kwamaprotheni, ekuhlungeni amaseli, neminye imikhakha.
I-Immunodiagnosis: Ubuhlalu be-Immunomagnetic bukhiwa izinhlayiya kazibuthe nezinto zokwakha ezinamaqembu asebenzayo. Ama-protein ligand (ama-antigens noma amasosha omzimba) ahlanganiswe ngokuhlanganyela emaqenjini asebenzayo obuhlalu kazibuthe, bese kwenziwa ukuhlolwa kwe-immunoassay kusetshenziswa i-magnetic bead protein complexes.
Ukuxilongwa kwamangqamuzana (i-nucleic acid extraction): Ubuhlalu obuzibuthe be-Nanoscale obunamaqembu angaphezulu angakha i-nucleic acid bungahlukaniswa futhi bukhangiswe yinkundla kazibuthe, bese buthululwa ukuze bathole isifanekiso se-nucleic acid.
Ukuhlanzwa kwamaprotheni: I-agarose exhumene ngokuhlanganyela ehlanganiswe nephrotheni ehlanganisiwe engu-A/G ebusweni bobuhlalu obuzibuthe, iphrotheni ethile ebophayo ye-ProteinA/G, futhi ekugcineni yakhishwa ukuze kutholwe amasosha omzimba ahlanzekile.
Ukuxilongwa Kwamasosha omzimba kanye nokuxilongwa kwe-Molecular:
Okunye okubalulekile kokusetshenziswa kobuhlalu kazibuthe kusekuhlolweni kwamasosha omzimba, lapho sekuphenduke amathuluzi abalulekile okuthola izifo okunembile. Isici esiyingqayizivele sobuhlalu kazibuthe sivela ekhonweni labo lokuthwebula nokuhlukanisa ama-antigen athile noma amasosha omzimba kumasampula esiguli, okwenza inqubo yokuxilonga ibe lula. Ngokuhlanganisa ngokuhlanganyela ama-protein ligands, njengama-antigens noma amasosha omzimba, emaqenjini asebenzayo obuhlalu kazibuthe, abacwaningi bangenza ama-immunoassay ngempumelelo nangokunemba okuthuthukisiwe.Ukuxilongwa kwamangqamuzana, enye insimu ethakazelisayo, izuza kakhulu ekusebenziseni ubuhlalu kazibuthe. Njengoba izindlela zokuxilonga zamangqamuzana zizuza ukuvelela eminyakeni yamuva nje, ubuhlalu obuzibuthe budlala indima ebalulekile ekuhlukaniseni nasekukhipheni ama-nucleic acid, njenge-DNA noma i-RNA, kumasampula ezinto eziphilayo. Lobu buhlalu busebenza njengezisekelo eziqinile, zenza kube lula ukuthwebula nokuhlanza ama-molecule okuhlosiwe. Le ndlela ethuthukisiwe yenze ososayensi bakwazi ukuthola ukuxilongwa okunembile nokuthembekile, okuholela emiphumeleni engcono yesiguli.
Ukuhlanzwa Kwamaprotheni Nokuhlelwa Kweseli:
Ubuhlalu obuzibuthe buphinde buthole ukusetshenziswa okubanzi ekuhlanzeni amaprotheni, inqubo ebalulekile ekuthuthukisweni kwezidakamizwa kanye nocwaningo lwe-biochemistry. Ngokuhlanganisa ama-ligand athile kubuhlalu, abacwaningi bangabopha ngokukhetha futhi bakhiphe amaprotheni okuhlosiwe anobucwebe obuphezulu kanye nesivuno. Le ndlela yokuhlanza isheshisa kakhulu inqubo yocwaningo iyonke, ivumela ososayensi ukuthi bahlaziye futhi bafunde amaprotheni ngendlela enemininingwane eyengeziwe.Ukuhlunga amaseli, okuyingxenye ebalulekile yezinhlelo ezihlukahlukene zezokwelapha nokucwaninga, kusengomunye umkhakha ozuzwa kakhulu ubuhlalu obukazibuthe. Lobu buhlalu, obuhlanganiswe nama-biomarker noma amasosha omzimba, asiza ukuhlukaniswa kanye nokuhlukaniswa kwamaseli ahlukene. Ngokusebenzisa amandla kazibuthe, ososayensi bangakwazi ukuhlela kahle futhi bahlukanise amaseli ngokusekelwe ezicini zabo ezingokomzimba nokusebenza kwazo. Ukunemba nokunemba kwale ndlela kuqinise imizamo yocwaningo ekuqondeni izinqubo eziyinkimbinkimbi zamaselula, njengokuqhubeka komdlavuza kanye nokusabela komzimba.
Isikhathi sokuthumela: Jun-25-2023