Emhlabeni we-automation yaselabhorethri, ukuthola izixazululo ezithuthukisa ukusebenza kahle nokunemba kubalulekile. Ngokufika kwepuleti elinesiketi esigcwele kahle esingu-96, abacwaningi nososayensi baye bavula amandla ezinga elisha lokuzenzakalelayo. Lawa mapuleti anikeza ububanzi bezici ezithuthukisa ukusebenza kokuhlaziya, ukuphepha kwesampula, nokuhlanganiswa okungenamthungo nezinhlelo zamarobhothi. Kule bhulogi, sizocubungula imininingwane yepuleti elinesiketi esigcwele kahle esingu-96 futhi sixoxe ngezinzuzo zalo zokusetshenziswa kwelabhorethri ehlukahlukene.
Thuthukisa ukusebenza kahle:
Enye yezinzuzo ezivelele zamapuleti angu-96 ane-skirt ngokugcwele yikhono lawo lokukhulisa ukusebenza kahle. Amapuleti adizayinelwe ukuthi alingane endaweni ejwayelekile ye-ANSI futhi ayabekeka kumasistimu azenzakalelayo, athuthukisa ukusetshenziswa kwendawo yelebhu ebalulekile. Abacwaningi manje bangenza inani elikhulu lokuhlola ngasikhathi sinye, bathuthukise kakhulu ukusebenza, ukukhiqiza, kanye nokusebenza ngempumelelo kwezindleko.
Thuthukisa ukusebenza kahle kwe-PCR:
Iphrofayili ephansi yepuleti elinesiketi esigcwele kahle esingu-96 lisiza ukunciphisa indawo efile futhi kuthuthukiswe ukusebenza kahle kwe-polymerase chain reaction (PCR). I-PCR iyindlela eyinhloko esetshenziselwa ukukhulisa i-DNA, futhi noma yikuphi ukuhlukahluka kwezinga lokushisa ngaphakathi kwepuleti kungase kubangele ukukhuliswa okungahambisani. Ukusetshenziswa kwalawa mapuleti kuqinisekisa ukudluliswa kokushisa okufanayo, kunciphisa amathuba okuhluka kwezinga lokushisa, futhi ekugcineni kwandisa ukuthembeka nokunemba kwemiphumela ye-PCR.
Ukuphatha irobhothi okuthuthukisiwe:
Ukuze kuhlanganiswe okungenamthungo nezinhlelo ezizenzakalelayo, ipuleti lesiketi esigcwele kahle esingu-96 sinikezwa njenge-superplate, eqinile ngokuphindwe kane. Lesi sici esibalulekile siqinisekisa ukuphatha okuhle kakhulu kwerobhothi futhi sinciphisa ubungozi bezingozi namaphutha ngesikhathi sokudluliselwa kwepuleti. Okokusebenza okuzenzakalelayo kuhamba ngendlela enokwethenjelwa, kuhlungwe futhi kubekwe kabusha amapuleti, okuholela ekusebenzeni okushelelayo nokunciphisa isikhathi sokuphumula.
Kuvalwe ngokuvikelekile ngaphandle kokuhwamuka:
Imiphetho ephakanyisiwe ezungeze umthombo ngamunye epuletini yenza kube lula ukuvala ukuhwamuka. Lolu phawu lubalulekile lapho uphatha amasampula abucayi adinga ukulawulwa okunembile kwevolumu nendawo. Abacwaningi bangaphumula kalula bazi ukuthi amasampula abo abalulekile avikelekile ekungcoleni nasekuhwamukeni, okuqinisekisa imiphumela yokuhlola engaguquki nethembekile.
Ukudlulisa Ukushisa Okungaguquki:
Ngokusebenzisa izindonga zomthombo ezacile ngokulinganayo, isiketi esigcwele kahle esingu-96 sinikeza ukudluliswa kokushisa okuphezulu nokungaguquguquki phakathi komthombo ngamunye. Lokhu kufana kubalulekile ezivivinyweni ezidinga ukulawulwa okunembile kwezinga lokushisa, njengokuhamba ngebhayisikili elishisayo, ukusabela kwe-enzymatic, kanye ne-protein crystallization. Amandla okudlulisa ukushisa asebenza kahle epuleti anika amandla imiphumela ethembekile nephinde ikhiqizeke, enciphisa ukuchema kokuhlolwa nokuthuthukisa ikhwalithi yedatha.
Isikhathi sokuthumela: Jun-25-2023