I-0.1ML ephezulu-skert e-96-kahle amapuleti

I-0.1ML inama-plats aphezulu aphezulu angama-96

Amapuleti ama-PCR 96 amathuluzi abalulekile asetshenziswa kwi-biology ye-molecular izicelo ezahlukahlukene, ikakhulukazi ekukhulisweni kwe-DNA ngokusebenzisa ukusabela kwe-polymerase (PCR). Nazi izinhlelo ezibalulekile:

1. I-DNA Amplification:
Ngokuyinhloko esetshenziselwa ukukhulisa amasampula we-DNA ezihlelweni zokuhlola okuphezulu, okuvumela amasampula amaningi ukuthi acutshungulwe ngasikhathi sinye.

2. Inani le-PCR (QPCR):
Ilungele i-PCR yesikhathi sangempela ye-PCR, inika amandla ubungako be-DNA noma i-RNA kwisampula kusetshenziswa amadayi noma ama-plues.

3. I-genotyping:
Oqashwe ezifundweni ze-genotyping ukuhlaziya ukuhlukahluka kofuzo kuwo wonke amasampula amaningi.

4. Ukuhlolwa kwe-Clone:
Iwusizo ekuhlolweni kwama-clones kuzivivinyo ze-molecular cloning, okuvumela abaphenyi ukuthi baqinisekise ukuba khona kokufakwa.

5. Izifundo zeMitagenesis:
Kusetshenziswe ezifundweni ezibandakanya i-mutagenesis eqondiswe yisayithi ukuhlaziya imiphumela yokuguqulwa okuthile kokuguqulwa okuthile kumsebenzi weGene.

6. Ukuhlola okuphezulu:
Kusiza izinzuzo zokusebenzisa okuphezulu, okwenza kufanelekile ukutholakala kwezidakamizwa kanye nezinye izinhlelo zokusebenza ezidinga ukuhlaziywa kwamasampula amaningi.

7. Isitoreji Sasesampula:
Ingasetshenziselwa ukugcinwa kwamasampula we-DNA noma ukuxutshwa kokuphendula ukuze kuhlaziywe kamuva.